We provide specialized Genome Editing Bioinformatics Tools that support the design, analysis, and optimization of genome editing experiments. These tools are essential for enhancing the precision, efficiency, and safety of genetic modifications across various organisms.

Our bioinformatics platforms are tailored for use with advanced genome editing technologies such as CRISPR/Cas, TALENs, and ZFNs, are invaluable in fields like gene therapy, functional genomics, agriculture, and synthetic biology.

Our Genome Editing Bioinformatics Capabilities Include:

•  Guide RNA (gRNA) Design and Optimization

Accurate design of guide RNAs with high on-target activity and minimal off-target effects for CRISPR applications.

•  Off-Target Prediction and Analysis

Genome-wide prediction of potential off-target sites to minimize unintended edits and improve specificity.

•  CRISPR/Cas Variant Selection Tools

Tools to choose the optimal Cas variant (e.g., Cas9, Cas12a) based on target sequence, delivery method, and editing goal.

•  Gene Knockout/Knock-in Strategy Design

Computational planning for precise gene disruption or insertion using homology-directed repair (HDR) or non-homologous end joining (NHEJ).

•  Primer Design for Validation Experiments

Automated primer generation for PCR validation of genome edits, including Sanger sequencing or NGS workflows.

•  Genome-Wide Target Screening

Identification of potential editing targets across the genome based on user-defined criteria.

•  Functional Impact Prediction

Analysis of how genome edits may affect gene function, protein structure, or regulatory regions.

•  Visualization Tools

Interactive genome browsers and editing maps to visualize gRNA positions, off-targets, and editing outcomes.

With our integrated bioinformatics support, Ayugenixz Bio Solutions helps researchers streamline genome editing projects, reduce trial-and-error, and accelerate the development of genetically engineered models and therapeutic interventions.